Wnt-3a

Human

HEK293 Cells (Fc)

Wnt Surrogate;Wnt Surrogate-Fc Fusion Protein;

Wnt-3a belongs to the Wnt family of signaling proteins, which plays an important role in maintaining the integrity of embryonic and adult tissues.The Wnt family is highly conserved and cysteine-rich.The Wnt signaling pathway can be divided into classical and non-classical pathways, and is involved in the β-catenin-mediated classical signaling pathway, including Wnt1, Wnt2, Wnt2b, Wnt3, Wnt3a, Wnt7a, Wnt8, Wnt8b and Wnt10a. Wnt3a, Wnt7a, Wnt8, Wnt8b and Wnt10a, the classical signaling pathway regulates stem cell pluripotent differentiation, organ development and regeneration.Wnt3a signals through the frizzled (FZD) family of receptors and the LRP5/6 co-receptor to regulate gene expression and a variety of cellular behaviors.Wnt3a, as the most representative signaling protein of the Wnt family, has a wide distribution, and has a role in the regulation of potent cellular functions (including self-renewal, proliferation, and cell regeneration). As the most representative signaling protein of the Wnt family, it is widely distributed and plays a key role in the regulation of pleiotropic cellular functions, including self-renewal, proliferation, differentiation, and motility.Wnt3a is an important ecological niche component for maintaining the proliferation of Lgr5-positive stem cells in intestinal epithelial cells. Currently, Wnt3a protein has been used in organoid cultures of intestinal, gastric, pancreatic, hepatic, thyroid, and ovarian cancers, and its high activity, batch-to-batch stability, and serum-free preparation are the keys to the success of organoid experiments.

60-75 kDa

> 95 % by SDS-PAGE analyses.

<0.1 EU/μg

Lyophilized in 0.22 μm filtered PBS solution (pH 7.4)

Brief centrifugation before opening is recommended to bring the contents to the bottom. Reconstitute to a concentration of 0.1-1.0 mg/mL in sterile distilled water. The stock solution should be divided into aliquots and stored at ≤ -20°C.

10, 100, 500μg, 1mg