Cat Number: 92575ES

Vimentin

Inquiry

Genus

Human

Source

E.coli

Synonyms

FLJ36605, VIM

Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis.

Molecular Weight

Approximately 53.5 kDa.

AA sequence

STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPSTSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA INTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQLKGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IMRLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQEEI AFLKKLHEEE IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQQYESVAA KNLQEAEEWY KSKFADLSEA ANRNNDALRQ AKQESTEYRR QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT IGRLQDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered solution in 30 % Acetonitrile and 0.1 % TFA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Note

100, 500μg