Source
E.coli(His-tagged)
Synonyms
Rat Tropic1808,Peptidoglycan hydrolase.
Description
Tropic1808 is a candidate chemotropic factor induced by nerve injury. TPC1808 protein, similar to NGF, could promote the expression of NF-H in a time-dependent manner. TPC1808 is the gene related to promotion of nerve growth, and both the TPC1808 gene and the TPC1808 recombinant protein up-regulate the expression of NF-H in PC12 cells.
Molecular Weight
Approximately 29.1 kDa
AA sequence
MSYYHHHHHH DYDIPTTENL YFQAMDPEFM NLAQIAALNQ ISNLNAIRVG QVLKVSNAAG SNNTQNTTQP SAGVPTNTAS STTGYTVKSG DTLSAIAAAN GVSLANLLSW NNLSLQAIIY PGQKLTIQNA NNATVTTPNA PTSTPTVMPS TNGSYTVKSG DTLYGIAAKL GTNVQTLLSL NGLQLSSTIY VGQVLKTTGA PVAGAGTATS TPTPVTPTVS KPAAANGVST AGLSAAQAAW LRTAVVDAQA ATAGTGVLAS VTVAQAILES GWGQSALASA PYHNFNLYLI KVKNTWKLMT LLLS
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.