Cat Number: 90621ES

TNF-α/TNFSF2-3

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Genus

Mouse

Source

E.coli

Synonyms

Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF

Description

Tumor necrosis factor alpha (TNF-α), also known as cachectin, is a cachectin, is a polypeptide cytokine produced by monocytes and macrophages. TNFα receptor signaling can simultaneously activate caspase 8, the transcription factor, NF-κB and the kinase, JNK. Human TNF-α shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. Inflammation is associated with insulin resistance, and TNF-α and IL-6 may affect glucose uptake. A wide variety of evidence has pointed to a critical role of TNF-α in tumour proliferation, migration, invasion and angiogenesis.

Molecular Weight

Approximately 17.4 kDa.

AA sequence

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg in the presence of actinomycin D. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Note

5, 20, 50, 500μg