TAFA-2

Human

E.coli

Chemokine-like protein TAFA-2, FAM19A2, MGC42403, TAFA2

TAFA2 also named FAM19A2, is a secreted, 11 kDa member of the FAM19/TAFA family of chemokine-like proteins. It is synthesized as a 131 amino acid (aa) precursor that contains a 30 aa signal sequence and a 101 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern CX7CCX13CXCX14CX11CX4CX5CX10C, where C represents a conserved cysteine residue and X represents any noncysteine amino acid. Human TAFA2 is 97% aa identical to mouse TAFA2. The biological functions of TAFA family members remain to be determined, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS. Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells. Finally, TAFAs may control axonal sprouting following brain injury.

Approximately 11.2 kDa.

ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H

Sterile Filtered White lyophilized (freeze-dried) powder.

>95% by SDS-PAGE and HPLC analyses.

The biological activity is determined by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. recombinant human TAFA-2, immobilized at 6-24 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth. Fully biologically active when compared to standard.

< 1.0 EU per 1μg of the protein by the LAL method.

Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

5, 100, 500μg