Cat Number: GMP-101-03

rHuIL-3 GMP

Inquiry

Source

Escherichia coli

Purity

> 98% by SDS-PAGE and HPLC analyses.

Molecular Weight

Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

Sequence

APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 U/mg

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 0.02% Tween-20, pH 7.0.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤ -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Refer to lot-specific CoA for the Expiry Date.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Endotoxin

Less than 0.01 EU/µg of rHuIL-3 GMP as determined by LAL method.

Host Cell Protein

Less than 0.05% when tested by ELISA

Host Cell DNA

Less than 20 ng/mg when tested by qPCR.

Reconstitution

Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Usage

This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory, or further evaluation purposes. NOT FOR HUMAN USE.

Quality Statement

The manufacturing and testing of these products comply with ICH Q7 guidelines.

Sterility

Negative

Mycoplasma

Negative

In Vitro Virus Assay

Negative

Standard

5μg, 100μg

Background

Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes, and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes, and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human, and murine IL-3 share low homology and it does not show activity on murine cells.