Purity
> 98% by SDS-PAGE and HPLC analyses.
Molecular Weight
Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
Sequence
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
Biological Activity
Measured in a cell proliferation assay using MCF 7 human breast cancer cells. The specific activity of recombinant human IGF-I/IGF-1 is >1000 IU/mg, which is calibrated against the human IGF-I/IGF-1 WHO International Standard (NIBSC code: 91/554).
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.0.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤ -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Refer to lot-specific CoA for the Expiry Date.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Endotoxin
Less than 0.01 EU/µg of rHuIGF-1 GMP as determined by LAL method.
Host Cell Protein
Less than 0.05% when tested by ELISA.
Host Cell DNA
Less than 20 ng/mg when tested by qPCR.
Reconstitution
Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Usage
This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory, or further evaluation purposes. NOT FOR HUMAN USE.
Quality Statement
The manufacturing and testing of these products comply with ICH Q7 guidelines.
In Vitro Virus Assay
Negative
Background
The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant human IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature human IGF-1 shares 94% and 96% a.a. sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.
