Cat Number: GMP-104-02

rHubFGF GMP

Inquiry

Source

Escherichia coli

Purity

> 98% by SDS-PAGE analyses.

Molecular Weight

Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids

Sequence

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 U/mg.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, 150 mM NaCl, 5% Trehalose, 0.02% Tween-20, pH 7.6.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤ -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Refer to lot-specific CoA for the Expiry Date.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Endotoxin

Less than 0.01 EU/µg of rHubFGF GMP as determined by LAL method.

Host Cell Protein

Less than 0.05% when tested by ELISA.

Host Cell DNA

Less than 20 ng/mg when tested by qPCR.

Reconstitution

Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Usage

This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory, or further evaluation purposes. NOT FOR HUMAN USE.

Quality Statement

The manufacturing and testing of these products comply with ICH Q7 guidelines.

Sterility

Negative

Mycoplasma

Negative

In Vitro Virus Assay

Negative

Standard

5μg, 100μg

Background

Human bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Human bFGF shares 54% amino acid sequence identity with aFGF. Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.