As the pharmaceutical and biotechnology industries continue to grow, the demand for innovative drug delivery systems (DDS) is increasing exponentially. The development of lipid nanoparticles (LNPs) is a promising technology that has received much attention. These versatile and customizable carriers have great potential for the effective delivery of a wide range of therapeutic agents from small molecules to biologics.

Protheragen-ING specializes in the development of lipid-based nanoparticles for various applications in the field of drug delivery. With our cutting-edge technology and expertise, we offer comprehensive services tailored to meet your specific needs. Our customized solutions will enable you to maximize the therapeutic potential of your drug candidates, ultimately providing more effective and safer treatments for your patients.

About LNP

LNPs are self-assembling colloidal structures composed of lipids such as phospholipids, cholesterol, and other lipid-like molecules. Conventional liposomes are characterized by a lipid bilayer surrounding an aqueous core. LNPs possess an electron-dense core in which ionizable cationic lipids are organized into anticolloid micelles around the encapsulated genetic material. Their ability to encapsulate and protect fragile drug payloads, coupled with their favorable pharmacokinetic profile and enhanced tissue targeting, make LNPs a preferred choice for a variety of therapeutic interventions.

Fig. 1 Lipid nanoparticle nucleic acid carriers: Nucleic acids are organized in antilipid micelles inside the nanoparticles. (Tenchov R, et al. 2021)

Advantages of Lipid-Based Nanoparticles as DDSs

• Their lipid composition closely mimics the natural lipid membranes found in cells, promoting effective cellular uptake and intracellular delivery of therapeutic agents.
• Lipid-based nanoparticles can encapsulate both hydrophilic and hydrophobic drugs, enabling simultaneous delivery of multiple active compounds. This versatility is particularly beneficial for combination therapy and targeted drug delivery.
• Lipid nanoparticles can protect the encapsulated drug from degradation, extend its circulation time in the body, and increase its bioavailability, thereby improving therapeutic efficacy.

Protheragen-ING's Comprehensive Development Services

• Formulation Design

We design lipid-based nanoparticle formulations to fit your specific therapeutic payload and delivery requirements. We optimize the formulation design by considering factors such as drug compatibility, stability, release kinetics, and target site.

• Lipid Selection and Optimization

Our extensive library of lipids and lipid derivatives allows us to select the most appropriate lipid composition for your nanoparticle formulation. Through systematic optimization, we can fine-tune the lipid composition for optimal drug loading, release profile, and stability.

• Nanoparticle Characterization

We employ a range of advanced characterization techniques to assess the physicochemical properties of the developed lipid nanoparticles, including particle size analysis, zeta potential measurements, determination of drug encapsulation efficiency, assessment of drug release kinetics, and stability testing.

• Nanoparticle Surface Modification

We offer surface modification services for lipid-based nanoparticles. Some of the common surface modification strategies include targeted modification, stability enhancement, and functionality introduction.

• In Vitro and In Vivo Evaluation

We conduct rigorous in vitro and in vivo studies to evaluate the performance and efficacy of nanoparticles, including cell culture assays to assess cellular uptake and cytotoxicity, as well as animal studies to investigate biodistribution, pharmacokinetics, and therapeutic outcomes.

Fig. 2 Approaches for organ specific modifications of LNPs used for RNA delivery. (Żak MM, et al. 2021)

Wide Range of Lipid-Based Nanoparticles

We can develop a wide range of lipid-based nanoparticle formulations based on your specific needs, including:

• Liposomes: Spherical vesicles composed of one or more lipid bilayers capable of encapsulating hydrophilic and hydrophobic drugs.
• Solid lipid nanoparticles (SLN): Nanoparticles made from solid lipids with enhanced stability and controlled drug release properties.
• Lipid-polymer hybrid nanoparticles: This system combines the advantages of lipids and polymers to provide enhanced encapsulation, targeting, and controlled release capabilities.
• Lipid-coated nanoparticles: Nanoparticles with lipid coatings that utilize the advantages of both lipid-based and polymer-based delivery systems.
• Lipid nanocapsules: Multifunctional core-shell structures with a liquid lipid core and a surrounding solid lipid shell for a variety of drug delivery applications.

Preparation Techniques for Lipid-Based Nanoparticles

We utilize advanced lipid nanoparticle preparation technologies to ensure the reproducibility, scalability, and quality of our formulations. Our manufacturing processes follow stringent quality control standards designed to optimize drug encapsulation efficiency, particle size distribution, and stability. Some of the methods we use include:

• High-pressure homogenization.
• Solvent evaporation.
• Thin film hydration.
• Microfluidization.

Contact with us today to unlock the full potential of lipid nanoparticles for pharmaceutical and biotechnology applications. Together, we'll tackle the complexities of drug delivery and drive successful clinical and commercial outcomes for your program.