Cat Number: 92542ES

Leptin

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Genus

Human

Source

E.coli

Synonyms

Obese Protein, Obesity Factor

Description

Leptin is a protein product of the mouse obese gene . It modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine, and pro-inflammatory immune responses. As a signal molecule with growth factor characteristics, leptin can stimulate osteoblastic cells and inhibit osteoclast formation and activity, thus promoting osteogenesis.

Molecular Weight

Approximately 16.0 kDa.

Purity

> 95% by SDS-PAGE.

Biological Activity

Immobilized Human Leptin Antibody at 3 μg/mL (100 μL/well) can bind Human Leptin , the EC50 of Human Leptin is 174-246 pg/mL.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1%BSA.

Reconstitution

1.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.
3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options. 5% trehalose is recommended for experimental culture without serum.

Note

100, 200μg, 1mg

HOURS

Daily: 9.30 AM–6.00 PM
Sunday : 9.30 AM–1.00 PM
Holidays: Closed

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