IL-36γ-1

Human

E.coli

IL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1

IL-36 gamma previously called IL-1F9, IL-1 epsilon (epsilon), and IL-1H1 is a member of the IL-1 family which includes IL1 beta, IL1 alpha, IL1ra, IL18, IL36 Ra (IL1F5), IL-36 alpha (IL1F6), IL36 beta (IL1F8), IL37 (IL1F7) and IL-1F10. All family members show a 12 beta strand, beta trefoil configuration, and are believed to have arisen from a common ancestral gene. IL36 gamma is an 1822 kDa, 169 aa intracellular and secreted protein that contains no signal sequence, no prosegment and no potential Nlinked glycosylation sites. Human IL36 gamma (aa 18169) shares 58%, 59%, 68% and 69% aa sequence identity with mouse, rat, bovine and equine IL36 gamma, respectively, and 2357% aa sequence identity with other family members. 134 aa isoform missing aa 1953 has been reported. Highest levels of IL36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to firstline defense against pathogens in the skin, lungs and digestive tract. Its expression is induced by LPS treatment of monocytes, and by IL alpha / beta, IL17 or TNF alpha treatment of keratinocytes and bronchial epithelia. Skin IL-36 gamma expression is increased in contact hypersensitivity and psoriasis. It is elevated in inflammatory disorders of the lung (such as asthma) and viral infections. Lung IL36 gamma and other IL36 proteins contribute to neutrophil influx. The receptor for IL-36 gamma is a combination of IL1 Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL1 RAcP. IL36 alpha, beta and gamma all activate NFkappa B and MAPK pathways in an IL1 Rrp2 dependent manner, and IL36 gamma induces production of inflammatory cytokines and chemokines such as CXCL8/IL8.

Approximately 17.0 kDa.

SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND

Sterile Filtered White lyophilized (freeze-dried) powder.

> 95% by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to standard. The ED50 as determined by its ability to induce IL-8 secretion by human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1 × 105 IU/mg.

< 1.0 EU per 1μg of the protein by the LAL method.

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

10, 100, 500μg