Cat Number: 90111ES

IL-12-1

Inquiry

Genus

Human

Source

Baculovirus

Synonyms

Interleukin-12, IL-12, NKSF2, CLMF p40

Description

Interleukin-12 (IL-12) is a cytokine that has regulatory effects on T and natural killer (NK) cells and is composed of two disulfide-bonded subunits, p40 and p35. It is a heterodimeric cytokine produced by macrophages, mitogen stimulated- or EBV infected-B lymphocytes, keratinocytes, and probably dendritic cells, with important immunoregulatory functions in vitro and in vivo. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS. Importantly, IL-12 also inhibited secondary granuloma formation in mice presensitized with eggs demonstrating a role for the cytokine in reversing established Th2-type responses.

Molecular Weight

Approximately 41 kDa and 29 kDa under reducing conditions.

AA sequence

HuIL-12 p40: Ile23 - Ser328; Accession # P29460
HuIL-12 p35: Arg23 - Ser219; Accession # P29459

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97 % by SDS-PAGE.

Biological Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rH IL-12 is approximately 1.1 × 104 units/μg, which is calibrated against rHuIL-12 WHO Standard (NIBSC code: 95/544).

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriately buffered solutions.

Note

2, 10, 100, 500μg