Cat Number: 92543ES

HBsAgadr

Source

CHO Cells

Synonyms

HBsAgadr; Hepatitis B virus surface antigen

Description

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.

Molecular Weight

Approximately 23.0-27.0 kDa on a reducing SDS-PAGE.

Appearance

Sterile liquid.

Purity

> 95 % by HPLC analyses.

Biological Activity

The biological activity is determined by ELISA with anti-HBsAg antibodies.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Sterile Filtered solution containing 20 mM PB, 154 mM NaCl.