Cat Number: 92547ES

HBsAg-preS2

Source

E.coli

Synonyms

Middle surface antigen,PreS2.

Description

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.

Molecular Weight

Approximately 5.7 kDa, a single non-glycosylated polypeptide chain containing 55 amino acids，with an N-terminal Met

AA sequence

MQWNSTTFHQ ALLDPKVRGL YFPAGGSSSG TVNPVPTTAS PISSIFSRTG DPAPN

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>95% by SDS-PAGE and HPLC analyses.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Note

10, 100, 500μg