eGFP

E.coli

Green Fluorecence Protein,GFP/SNAP25B/VAMP-2

Green fluorescent protein (GFP) refers to a protein purified from the Victorian jellyfish that displays green fluorescence when excited by short-wave light (blue to ultraviolet). Although wild-type GFP exists, many GFP mutants have been adapted for broader research uses. Green fluorescent protein (GFP) can be genetically modified to form fusion proteins with a number of proteins while maintaining the activity of the target protein and the fluorescence of GFP. GFP has been used as a reporter gene for gene expression in cellular and molecular biology and as a fusion tag for localization studies of proteins in living cells. it can also be used as a molecular thermometer to accurately measure the temperature of a fluid. Enhanced GFP (eGFP) has the S65T and F64L mutations, which cause GFP to exhibit enhanced fluorescence at 37°C, respectively. Recombinant Enhanced Green Fluorescent Protein (EGFP) is a non-glycosylated, homodimeric protein expressed in Escherichia coli that contains 239 amino acids and has a molecular weight of 26.9 kDa.

Approximately 26.9 kDa, a single non-glycosylated polypeptide chain containing 39 amino acids.

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

Sterile Filtered White lyophilized (freeze-dried) powder.

> 95% by SDS-PAGE and HPLC analyses.

< 1.0 EU per 1μg of the protein by the LAL method.

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

It is recommended that the vial be centrifuged briefly before opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further depending on subsequent experiments; for long-term storage, the addition of carrier protein (0.1% BSA) is recommended; dispense and avoid repeated freezing and thawing.

10, 100μg, 1mg