Cat Number: 90926ES

CXCL9-2

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Genus

Mouse

Source

E.coli

Synonyms

C-X-C motif chemokine 9, CXCL9, Gamma-interferon-induced Monokine, Humig, MIG, Small-inducible Cytokine B9

Description

CXCL9 is a member of the CXC family and has an important role in the chemotaxis of immune cells. The mouse CXCL9 shares 75% and 88% a.a. sequence identity with human and rat CXCL9. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells.

Molecular Weight

Approximately 12.2 kDa.

AA sequence

TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/mL. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Note

5, 100, 500μg