Cat Number: 90945ES

CXCL4-3

Genus

Bovine

Source

E.coli

Synonyms

chemokine (C-X-C motif) ligand 4, C-X-C motif chemokine 4, CXCL4, CXCL4iroplact, Iroplact, MGC138298, Oncostatin-A, PF4, platelet factor 4

Description

CXCL4, also called PF4, is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand. Mature mouse CXCL4 shares 76%, 88%, 64%, 64% and 63% amino acid sequence identity with human, rat, ovine, porcine and bovine CXCL4, respectively. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. CXCL4 can be antiproliferative and antiangiogenic, at least in part via interfering with FGF-2 and VEGF heparin binding and thus inhibiting their signaling. Tumor tissue revealed up-regulation of CXCL14 in cancer-associated fibroblasts of a majority of prostate cancer. Fibroblasts overexpressing CXCL14 promoted the growth of prostate cancer xenografts, and increased tumor angiogenesis and macrophage infiltration.

Molecular Weight

Approximately 9.5 kDa.

AA sequence

ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDES

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml. Fully biologically active when compared to standard.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Note

10, 100, 500μg