Cat Number: 90996ES

CXCL17-2

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Genus

Rat

Source

E.coli

Synonyms

Dendritic Cell and Monocyte Chemokine-like Protein, DMC

Description

Dendritic cell and monocyte chemokine-like protein (DMC), also known as VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK. It has no predicted N-glycosylation sites, so cleavage of a 22 amino acid (aa) signal sequence likely results in a mature mouse DMC molecule of 97 aa and 11 kDa size. DMC is constitutively produced by airway and intestinal epithelium. It induces the chemotaxis of quiescent, but not LPSactivated peripheral blood monocytes and dendritic cells, and also binds these cells specifically.

Molecular Weight

Approximately 11.5 kDa

AA sequence

SPNQEVARHH GDQHQAPRRW LWEGGQECDC KDWSLRVSKR KTTAVLEPPR KQCPCDHVKG SEKKNRRQKH HRKSQRPSRT CQQFLKRCQL ASFTLPL

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by its ability to induce VEGF expression using murine endothelial cells is less than 5.0 μg/ml, corresponding to a specific activity of > 200 IU/mg.

Endotoxin

<0.1 EU/μg of protein as determined by LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Note

10, 100, 500μg