Cat Number: 90932ES

CXCL15

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Genus

Mouse

Source

E.coli

Synonyms

CXCL15, Il8, IL-8, Lungkine, Scyb15, Weche

Description

Mouse Lungkine/CXCL15, also named WECHE, is a member of the ELR motif-containing CXC chemokines. Mouse Lungkine shares 35% aa sequence identity with human ENA-78 and 31% identity with human IL-8. CXCL15 is a novel CXC chemokine that is highly expressed in the adult mouse lung. CXCL15 has suppressive activity on proliferation and expansion of multi-potential, erythroid, granulocyte and macrophage progenitors in S-phase specific manner. Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking. Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation.

Molecular Weight

Approximately 16.4 kDa.

AA sequence

QELRCLCIQE HSEFIPLKLI KNIMVIFETI YCNRKEVIAV PKNGSMICLD PDAPWVKATV GPITNRFLPE DLKQKEFPPA MKLLYSVEHE KPLYLSFGRP ENKRIFPFPI RETSRHFADL AHNSDRNFLR DSSEVSLTGS DA

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 20-100 ng/mL. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 0.02% Tween-20.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Note

5, 100, 500μg