Cat Number: 90929ES

CXCL12α-2

Genus

Mouse

Source

E.coli

Synonyms

CXCL12 chemokine (C-X-C motif) ligand 12, CXCL12/SDF-1 alpha, IRH, PBSF, SCYB12, SDF1 alpha, SDF1, SDF1a, SDF1B, TLSF, TPAR1

Description

CXCL12 is belonging to the CXC chemokine family. SDF_x005f_x005f_x005f_x001e_1 alpha and SDF1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 is highly conserved between species, mouse CXCL12α shares approximately 93% amino acid sequence identity with human CXCL12α. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail.

Molecular Weight

Approximately 8.0 kDa.

AA sequence

KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/mL. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Note

10, 100, 500μg