Cat Number: 90937ES

CINC-2β/CXCL3

Inquiry

Genus

Rat

Source

E.coli

Synonyms

chemokine (C-X-C motif) ligand 3, CINC-2beta, MIP2-beta

Description

CXCL3, also known as DCIP1 in mouse, CINC2 in rat, and GROγ in humans, is belonging to the CXC chemokine family. The amino acid sequence of mouse CXCL3 is 57 % identical to human CXCL3. In rat, CINC-2 has two isoforms, known as CXCL3α/CINC-2α and CXCL3β/CINC-2β. CXCL3 plays a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. CXCL3 was reportedly associated with the invasion and metastasis of various malignancies. As a member of the chemokine family, CXCL3 was previously known to participate in many pathophysiological events. CXCL3 was found to be upregulated in aggressive cancer cells.

Molecular Weight

Approximately 7.6 kDa.

AA sequence

RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSPSL

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/ml. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Note

10, 100, 500μg