Cat Number: 91005ES

CCL28-3

Inquiry

Genus

Rat

Source

E.coli

Synonyms

Rat MEC/CCL28

Description

CCL28 (CC chemokine ligand 28)，as know as mucosae-associated epithelial chemokine (MEC), is a novel CC chemokine. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The CCL28 gene has been mapped to the distal region of chromosome 13. CCL28 is produced by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum . The protein signals through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils.

Molecular Weight

Approximately 13.1 kDa.

AA sequence

SEAILPIASS CCTEVSHHIP RRLLERVNSC SIQRADGDCD LAAVILHVKR RRICVSPHNP TLKRWMSASE MKNGKENLCP RKKQDSGKDR KGHTPRKHGK HGTRRIHGTH DHEAPR

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Biological Activity

The biologically active determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 5.0-50 ng/mL. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 200 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Note

5, 100, 500μg