CCL2

Human

E.coli

HC11, Monocyte chemoattractant protein 1, MCAF, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2

CCL2, also called monocyte chemotactic protein1 (MCP1) or JE, is a member of the CC or beta chemokine family that is best known as a chemotactic agent for mononuclear cells . Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein . Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acidmodified glutamine, occurs naturally by metalloproteinase cleavage and downregulates activity but not receptor binding. CCL2 may form multiple bands from 8.713.5 kDa on SDSPAGE due to noncovalent dimerization and variable carbohydrate content. Mature human CCL2 shares 7879% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only 56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells.

Approximately 8.7 kDa

QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

Sterile Filtered White lyophilized (freeze-dried) powder.

> 96% by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL.

< 1 EU per 1μg of the protein by the LAL method.

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

5, 20, 50, 100μg