Cat Number: 90963ES

CCL17-1

Inquiry

Genus

Human

Source

E.coli

Synonyms

MGC138273, SCYA17, SCYA17MGC138271

Description

CCL17, also known as thymus and activation-related chemokine (TARC), a small cytokine belonging to the C-C chemokine family. Among CC chemokine family members, CCL17 has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in phytohemagglutinin-stimulated peripheral blood mononuclear cells. CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

Molecular Weight

Approximately 8.1 kDa.

AA sequence

ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/mL. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Note

5, 100, 500μg