Cat Number: 90959ES

CCL14

Genus

Human

Source

E.coli

Synonyms

CC-1 Protein, Human; CC-3 Protein, Human; CKB1 Protein, Human; FLJ16015 Protein, Human; HCC-1 Protein, Human; HCC-1(1-74) Protein, Human; HCC-1/HCC-3 Protein, Human; HCC-3 Protein, Human; MCIF Protein, Human; NCC-2 Protein, Human; NCC2 Protein, Human; SCYA14 Protein, Human; SCYL2 Protein, Human; SY14 Protein, Human

Description

Human CCL14 is belonging to the CC chemokine family. It has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. CCL14 is expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations in plasma.

Molecular Weight

Approximately 8.4 kDa.

AA sequence

TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Note

2, 10, 50, 100μg