Cat Number: 92053ES

BMP-4

Inquiry

Genus

Human

Source

E.coli

Synonyms

BMP2B, BMP-2B, BMP2B1, BMP2BMCOPS6, BMP4,DVR4, MCOPS6, OFC11, ZYME

Description

Bone morphogenetic protein-4 (BMP-4) is one of nine structurally related BMPs belonging to the transforming growth factor-beta (TGF-beta) superfamily of secreted proteins. The human BMP-4 shares 98 % sequence indentity with mouse BMP-4. Mature BMP-4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP-4, a stimulator of chondrogenesis, both in vitro and in vivo, is a potential therapeutic agent for cartilage regeneration.

Molecular Weight

The recombinant Human BMP4 predicts a molecular mass of 13.25 kDa.

Purity

> 95% by SDS-PAGE

Biological Activity

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 0.08-0.4ug/mL.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from sterile 30% Acetonitrile, 1/1000 TFA. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Note

20, 100μg

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Daily: 9.30 AM–6.00 PM
Sunday : 9.30 AM–1.00 PM
Holidays: Closed

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