BMP-2

Human

E.coli

BDA2, BMP2, BMP-2, BMP-2A, SSFSC

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup, of which BMP2-7 belongs to the TGF-β superfamily. It plays a dominant role in embryonic dorsoventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. Mature human BMP-2 has 100% aa sequence identity with mouse and rat BMP-2. It has 85% sequence identity to human BMP-4 and less than 51% sequence identity to other BMPs. Normal bone formation is a carefully regulated long-term process involving the continuous expression of growth regulators by osteoblasts during proliferation and eventual differentiation. Because this ordered sequence of osteoblast gene expression suggests a cascade effect, BMP-2 is able to initiate and maintain this effect. BMP-2 also promotes maintenance and repair of colonic epithelium, inhibits neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for myocardial contractility.

Approximately 26.0 kDa.

Sterile Filtered White lyophilized (freeze-dried) powder.

>95 % by SDS-PAGE and HPLC analyses.

The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/mL, corresponding to a specific activity of > 5.0 × 103 IU/mg. Fully biologically active when compared to standard.

< 1.0 EU per 1μg of the protein by the LAL method.

Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM sodium citrate, pH 3.5.

Brief centrifugation before opening is recommended to bring the contents to the bottom. Redissolve in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Aliquot reserve solution and store at ≤ -20°C. Perform further dilutions in appropriate buffer solutions. Do not reconstitute in PBS or cell culture media.

10, 100, 500μg